Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

1.03840

Cellvento® 4CHO-X COMP

expansion medium

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice

About This Item

UNSPSC Code:
41106213
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

500

description

shipped with cooling packs

product line

Cellvento®

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

storage temp.

2-8°C

General description

There are several advantages to using perfusion in your seed train, namely, flexibility in your production processes, increase in capacity and footprint reduction. Reducing the number of bioreactors in your seed train leads to a better utilization and eliminates traditional bottlenecks (compressed seed train). Additionally, using N-1 perfusion enables the inoculation of a production bioreactor with higher cell densities for high seed production process formats. Cellvento® 4CHO-X Expansion Medium has been specifically developed for N-1 perfusion for Chinese Hamster Ovary (CHO) cell lines. It is formulated with a high nutrient concentration to achieve low cell specific perfusion rates (CSPRs) at high cell densities. The medium is chemically defined, of non-animal origin, and contains no hydrolysates or components of unknown composition.

Application

Cellvento® 4CHO-X Expansion Medium has been specifically formulated to control the metabolic profile of the cells, both to minimize toxic byproducts and to ensure adequate nutrient levels for high cell densities. Preventing nutrient depletion can increase robustness and predictability of seed train operations. This medium supports high growth and viability, thus increasing cell biomass for cryopreservation or inoculation of another reactor. The medium addresses seed train intensification and shows a positive impact in IgG production, when combined in perfusion production with EX-CELL® Advanced HD Perfusion medium (24370C), and in Fed-batch production with e.g. Cellvento® 4CHO medium (103795) and Cellvento® 4Feed (103796). It is expected to work with other media platforms as well. Cellvento® 4CHO-X Expansion Medium can be used for all seed train expansion steps including N-1, reaching robust growth at low CSPRs. This product is intended for research or further manufacturing but not for human or therapeutic use.

Preparation Note

Formulated to contain 26.2 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
1.0379514390C1.03796
Cellvento® 4CHO-X COMP expansion medium

SAFC

1.03840

Cellvento® 4CHO-X COMP

Cellvento® 4CHO COMP chemically defined cell culture medium, ≤1.0 IU/mL endotoxin, pH 5.1-5.6(without supplement), pH 6.7-7.3(with supplement)

SAFC

1.03795

Cellvento® 4CHO COMP

Cellvento® 4CHO-C Cloning Medium Animal- component free, chemically defined

SAFC

14390C

Cellvento® 4CHO-C Cloning Medium

Cellvento® 4Feed COMP chemically defined cell culture feed

SAFC

1.03796

Cellvento® 4Feed COMP

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

form

dry powder

form

dry powder

form

liquid

form

dry powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

product line

Cellvento®

product line

Cellvento®

product line

Cellvento®

product line

Cellvento®

Quality Level

500

Quality Level

500

Quality Level

-

Quality Level

500

description

shipped with cooling packs

description

shipped with cooling packs

description

-

description

shipped with cooling packs

Stay compliant with GMP using the Emprove® Program

Accelerate qualification, maintain compliance, and save time with Emprove® Dossiers.

Download Dossiers

Note: you will be brought to EmproveSuite.com. Your username and password are separate from SigmaAldrich.com and may differ.

Register for free and access Emprove® Dossiers within minutes. Upgrade your subscription plan to unlock advanced and customized content in the Emprove® Suite

Not finding what you are looking for? Visit the Emprove® Program Help page.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Operational and Financial Advantages of Cellicon® Cell Retention Solution in an N-1 Perfusion Process

This page describes the financial and operational advantages offered by the Cellicon® Cell Retention Device in an N-1 perfusion process at the 2,000 L scale in comparison to a cell retention device from another supplier.

Reproducible Scaling of a Cell Retention Filter Using the Cellicon® Cell Retention Solution

This page describes the design attributes of the Cellicon® filter assembly for cell retention and how they simplify scale-up of intensified upstream processes.

Cell Retention Technology and Specialized Cell Culture Media for Intensified Upstream Processing

How the Cellicon® filter and controller enables seamless adoption of a perfused N-1 seed train step into the cell expansion process.

View All Articles

Related Content

Successfully Navigate the Regulatory Landscape for Chemicals and Single-Use Consumables

This page summarizes key considerations related to regulatory expectations for biomanufacturing including chemicals and polymeric materials. For chemicals, topics include nitrosamines and elemental impurities, and for polymeric materials such as filters and single-use assemblies, the focus is qualification and extractable and leachable testing.

Cell Culture Media Development for mAbs

Learn more about cell culture media development for mAB development and manufacturing, and the products you need for this step.

Global Trade Item Number

SKUGTIN
103840050004054839967641
103840010004054839754036
103840000104054839754050
103840001004054839754043
103840000304065272075381

Questions

Reviews

No rating value

Active Filters