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GranuCult® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar

acc. FDA-BAM, ISO 21872

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Synonym(s):
TCBS Agar, Thiosulfate Citrate Bile Salts Sucrose Agar, Vibrio Selective Agar

agency

ISO 21872

Quality Level

form

powder

technique(s)

microbiological culture: suitable

pH

8.6±0.2 (25 °C)

application(s)

food and beverages
pharmaceutical

storage temp.

15-25°C

suitability

selective and differential for Vibrio spp.

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1.161221.002151.05287
suitability

selective and differential for Vibrio spp.

suitability

Escherichia coli

suitability

selective and differential for Salmonella spp., selective and differential for Shigella spp.

suitability

Salmonella spp.

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

agency

ISO 21872

agency

ISO 16649

agency

FDA-BAM, according to ISO 21567, according to ISO 6579

agency

APHA, ISO 19250, ISO 6579

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

food and beverages
pharmaceutical

application(s)

food and beverages, microbiology

application(s)

clinical
food and beverages
industrial qc
pharmaceutical, microbiology

application(s)

food and beverages, microbiology

General description

GranuCult® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar is a complex medium designed for the isolation and differentiation of Vibrio spp. and other enteropathogenic microorganisms from products intended for human consumption, animal feed, environmental samples in the production area, food production, food handling, and other materials.
The synergistic effect of high concentration of thiosulfate, citrate and strong alkalinity inhibits the growth of Enterobacteriaceae spp. The medium is highly selective as the bile salts present in the medium suppresses the growth of Enterococci spp.
The differentiation is achieved based on sucrose fermentation, pH indicators and H2S production from thiosulfate. Vibrio spp. do not produce H2S but depending on the species, may ferment sucrose. Peptone and yeast extract in the media allow good growth and agar is the solidifying agent.

Application

GranuCult® prime Thiosulfate Citrate Bile Sucrose (TCBS) Agar is used to isolate and differentiate Vibrio spp. and other enteropathogenic microorganisms from products intended for human consumption, animal feed, environmental samples in the production area, food production, food handling, and other materials.

Analysis Note

  • The dehydrated medium is a granulate with green color.
  • The prepared medium is clear to slightly opalescent and blue-green.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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