1.03871
GranuCult® prime CIN (Cefsulodin, Irgasan, Novobiocin) Agar
acc. SCHIEMANN, ISO 10273
Synonym(s):
CIN Agar, Cefsulodin, Irgasan, Novobiocin Agar, SCHIEMANN’S Agar, Yersinia Selective Agar (YSA)
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About This Item
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agency
ISO 10273
Quality Level
form
powder
technique(s)
microbiological culture: suitable
pH
7.4±0.2 (25 °C)
application(s)
food and beverages
pharmaceutical
storage temp.
15-25°C
suitability
selective for (Microorganism: Yersinia)
1 of 4
This Item | 1.03857 | 1.00215 | 1.03854 |
|---|---|---|---|
| agency ISO 10273 | agency ISO 21150 | agency FDA-BAM, according to ISO 21567, according to ISO 6579 | agency ISO 21872 |
| form powder | form powder | form granular | form powder |
| suitability selective for (Microorganism: Yersinia) | suitability selective and differential for enterobacteriaceae | suitability selective and differential for Salmonella spp., selective and differential for Shigella spp. | suitability selective and differential for Vibrio spp. |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| application(s) food and beverages | application(s) food and beverages | application(s) clinical | application(s) food and beverages |
| pH 7.4±0.2 (25 °C) | pH 7.1±0.2 (25 °C) | pH 7.5-7.9 (25 °C, 75 g/L in H2O) | pH 8.6±0.2 (25 °C) |
General description
The selectivity of the medium is contributed using antibiotics- Cefsulodin, Irgasan(Triclosan), Novobiocin, bile salts and crystal violet dye which synergistically inhibit the accompanying flora. The pyruvate in the medium promotes the growth of Yersinia spp. agar is the solidifying agent.
Mannitol from the medium is degraded by the growing Yersinia to form an acid which will trigger a pH change indicated by neutral red. The positive colonies appear red in color. The fermentation reaction by the growing Yersinia is intense and localized which produces the characteristic “bull′s eye” appearance. The microbes which do not metabolize mannitol from the media will form colorless, translucent colonies.
Application
Analysis Note
- The antibiotics- Cefsulodin, Irgasan(Triclosan), Novobiocin are added separately after autoclaving.
- The dehydrated medium is granulate with pink color.
- The prepared medium is clear to slightly opalescent and red.
Footnote
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
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Warning
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Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
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