MilliporeSigma
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1.04057

Supelco

Glycerol

greener alternative

(vegetable origin) for analysis EMSURE® ACS,Reag. Ph Eur

Synonym(s):
1,2,3-Propanetriol, Trihydroxylpropane, Protol, Glycerin, 1,2,3-Propanetriol, Glycerin
Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
Molecular Weight:
92.09
Beilstein:
635685
MDL number:
EC Index Number:
200-289-5

Quality Level

grade

ACS reagent

Agency

reag. Ph. Eur.

vapor density

3.1 (vs air)

vapor pressure

<1 mmHg ( 20 °C)

product line

EMSURE®

assay

≥99.5% (GC)
98.0-101.0% (calculated on anhydrous substance, alkalimetric)

form

liquid

autoignition temp.

698 °F

expl. lim.

2.6-11.3 % (v/v)

greener alternative product characteristics

Safer Solvents and Auxiliaries
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

technique(s)

electrophoresis: suitable

impurities

≤0.001% Chloride (Cl)
≤0.001% Sulfate (SO4)
≤0.002% Free acid (CH3COOH)
≤0.003% Halogenated compounds (as Cl)
≤0.05% Fatty acids and esters (as Butyric acid)
≤0.1% Diethylene glycol and related Substances (any further impurity)
≤0.1% Diethylene glycol and related Substances (impurity A)
≤0.5% Diethylene glycol and related Substances (Sum of all impurities)
≤0.5% Water
≤10 ppm Aldehydes
≤3000 ppm Methanol

color

APHA: ≤10

refractive index

n20/D 1.474 (lit.)

pH

5 (20 °C, 100 g/L in H2O)

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

transition temp

flash point 199 °C

density

1.25 g/mL (lit.)

cation traces

Al: ≤0.00005%
B: ≤0.000002%
Ba: ≤0.00001%
Ca: ≤0.0001%
Cd: ≤0.000005%
Co: ≤0.000005%
Cr: ≤0.000002%
Cu: ≤0.000005%
Fe: ≤0.00001%
Mg: ≤0.0001%
Mn: ≤0.00001%
Ni: ≤0.000002%
Pb: ≤0.00001%
Sn: ≤0.00001%
Zn: ≤0.00001%
heavy metals (as Pb): ≤0.00020%

SMILES string

OCC(O)CO

greener alternative category

storage temp.

2-30°C

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Analysis Note

Assay (GC, calc. on anhydr. substance): ≥ 99.5 %
Assay (alkalimetric, calculated on anhydrous substance): 98.0 - 101.0 %
Identity: passes test
Color: ≤ 10 APHA
Appearance of solution: passes test
Acidity or alkalinity: passes test
Free acid (CH₃COOH): ≤ 0.002 %
Neutrality: passes test
Refractive index (n 20/D): 1.470 - 1.475
Chloride (Cl): ≤ 0.0010 %
Sulfate (SO₄): ≤ 0.001 %
Halogenated compounds (as Cl): ≤ 0.0030 %
Heavy metals (as Pb): ≤ 0.00020 %
Al (Aluminium): ≤ 0.00005 %
B (Boron): ≤ 0.000002 %
Ba (Barium): ≤ 0.00001 %
Ca (Calcium): ≤ 0.0001 %
Cd (Cadmium): ≤ 0.000005 %
Co (Cobalt): ≤ 0.000005 %
Cr (Chromium): ≤ 0.000002 %
Cu (Copper): ≤ 0.000005 %
Fe (Iron): ≤ 0.00001 %
Mg (Magnesium): ≤ 0.0001 %
Mn (Manganese): ≤ 0.00001 %
Ni (Nickel): ≤ 0.000002 %
Pb (Lead): ≤ 0.00001 %
Sn (Tin): ≤ 0.00001 %
Zn (Zinc): ≤ 0.00001 %
Methanol: ≤ 3000 ppm
Other residual solvents (ICH Q3C): ...excluded by production process
Acrolein and glucose: passes test
Aldehydes: ≤ 10 ppm
Diethylene glycol and related Substances (impurity A): ≤ 0.1 %
Diethylene glycol and related Substances (any further impurity): ≤ 0.1 %
Diethylene glycol and related Substances (Sum of all impurities): ≤ 0.5 %
Fatty acids and esters (as Butyric acid): ≤ 0.05 %
Substances darkened by sulfuric acid: passes test
Sugar: passes test
Sulfated ash: ≤ 0.005 %
Water: ≤ 0.5 %

Legal Information

EMSURE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

390.2 °F - Pensky-Martens closed cup

Flash Point(C)

199 °C - Pensky-Martens closed cup

Certificate of Analysis

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Certificate of Quality

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