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Sigma-Aldrich

Methylene blue (C.I.52015)

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Synonym(s):
Methylene Blue trihydrate
Empirical Formula (Hill Notation):
C16H18ClN3S · 3H2O
CAS Number:
Molecular Weight:
373.90
Beilstein/REAXYS Number:
3599847
MDL number:

form

solid

Quality Level

potency

1180 mg/kg LD50, oral (Rat)

composition

Dye, ≥82% spectrophotometric assay

pH

3 (20 °C, 10 g/L in H2O)

mp

190 °C (dec.) (lit.)

solubility

50 g/L

density

1.757 g/cm3 at 22 °C

bulk density

400‑600 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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Methylene blue (C.I.52015) for microscopy Certistain®

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Methylene blue (C.I.52015)

Methylene Blue Trihydrate OmniPur® Grade

6210-OP

Methylene Blue Trihydrate

solubility

50 g/L

solubility

50 g/L

solubility

50 g/L

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

composition

Dye, ≥82% spectrophotometric assay

composition

-

composition

Dye, ≥82% spectrophotometric assay

composition

-

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

100

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

-

General description

Methylene blue (C.I. 52015) for microscopy Certistain® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain®

Analysis Note

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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