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1.04137

Sigma-Aldrich

Crystal violet (C.I. 42555)

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Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
Molecular Weight:
407.98
MDL number:

form

solid

Quality Level

potency

420 mg/kg LD50, oral (Rat)

composition

Dye, ≥88% spectrophotometric assay

pH

2.5-3.5 (20 °C, 10 g/L in H2O)

mp

189-194 °C

solubility

10 g/L

density

1.19 g/L at 20 °C

bulk density

220‑400 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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1 of 4

This Item
1.014083267549144
vibrant-m

1.04137

Crystal violet (C.I. 42555)

vibrant-m

1.01408

Crystal violet (C.I. 42555)

vibrant-m

32675

Crystal Violet

vibrant-m

49144

Gentian Violet solution

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

room temp

storage temp.

room temp

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

solid

form

solid

form

powder

form

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

density

1.19 g/L at 20 °C

density

1.19 g/cm3 at 20 °C

density

1.190 g/cm3

density

-

General description

Crystal violet (C.I. 42555) for microscopy Certistain®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain®

Analysis Note

Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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