Nuclear fast red (C.I. 60760) for microscopy Certistain®, is a dry dye for microscopy that is often used for staining nuclei in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of a Nuclear fast red staining solution. It is commonly used for counterstaining in histochemical reactions, e.g. in Berlin blue staining for detection of iron or in silver plating of reticulin fibers, as well as nuclear staining with Alcian blue. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.
Application
for microscopy Certistain®
Analysis Note
Dye content (spectrophotometrically): ≥ 90 % Identity - sodium: passes test - UV/VIS-Spectrum: passes test Absorption maximum λ 1 (Dimethyl sulfoxide): 557 - 559 nm Absorption maximum λ 2 (Dimethyl sulfoxide): 520 - 522 nm Spec. Absorptivity A 1%/1cm - λ1max; 0.02 g/l; dimethyl sulphoxide: 380 - 422 - λλ2max; 0.02 g/l; Dimethyl sulphoxide: 383 - 426 TLC-Test: passes test Loss on drying (110 °C): ≤ 5 % Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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