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1.04178

Sigma-Aldrich

Rhodamine B (C.I.45170)

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Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
Molecular Weight:
479.01
MDL number:

form

solid

Quality Level

potency

>2000 mg/kg LD50, oral (Rat)

composition

Dye, ≥90% spectrophotometric assay

pH

3-4 (20 °C, 10 g/L in H2O)

mp

199-201 °C

solubility

15 g/L

density

1.31 g/cm3 at 20 °C

bulk density

250 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

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1 of 4

This Item
R66267975483689
Sigma-Aldrich

1.04178

Rhodamine B (C.I.45170)

Rhodamine B ≥95% (HPLC)

R6626

Rhodamine B

Rhodamine B analytical standard

79754

Rhodamine B

Rhodamine B for fluorescence

83689

Rhodamine B

form

solid

form

powder

form

-

form

powder or crystals

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
food and beverages
personal care

application(s)

-

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

-

composition

Dye, ≥90% spectrophotometric assay

composition

-

composition

-

composition

-

storage temp.

2-30°C

storage temp.

room temp

storage temp.

-

storage temp.

-

General description

Rhodamine B (C.I. 45170) for microscopy, is a solid dye for fluorescence AFB staining of bacteriological smears and histological tissue sections of human origin. The mechanism is a non-specific binding of fluorescence dye on a substrate, similar to the classical Ziehl-Neelsen method, although the heating of the Rhodamine B color solution can be avoided. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 550 - 552 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2115 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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