Brilliant cresyl blue zinc chloride double salt for microscopy Certistain®, is a dry staining dye that is used to prepare a staining solution, that makes target structures (by staining with the above brilliant cresyl blue solution) evaluable for diagnostic purposes. The regenerative capacity of erythrocytes can be detected by counting of reticulocytes. The measurement of substantia granulofilamentosa (ribonucleoproteins) is possible with fresh, non-fixed, young erythrocytes (supravital staining).Four stages of substantia granulofilamentosa maturation can be distinguished depending on the stage of reticulocyte development: coiled skein (I), incomplete network (II), complete network (III) and granular form (IV). In peripheral blood the development stages III and IV are found most commonly. When stained with brilliant cresyl blue the reticulocytes display a black-blue network or black-blue dots. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.
Application
for microscopy Certistain®
Analysis Note
Dye content (spectrophotometrically): ≥ 60% C₁₇H₂₀N₃O Identity - UV/VIS-Spectrum: passes test - Zinc: passes test Absorption maximum λ max (ethanol 50 %): 623 - 628 nm Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; ethanol 50 %): ≥ 1500 TLC-Test: passes test Loss on drying (110 °C): ≤ 5 % Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
