1.04208

Brilliant green (hydrogen sulfate) (C.I. 42040)

• Empirical Formula (Hill Notation): C₂₇H₃₄N₂O₄S
• CAS Number: 633-03-4
• Molecular Weight: 482.63
• NACRES: NA.21
• UNSPSC Code: 41116130
• MDL number: MFCD00011880

Properties

• form: solid
• Quality Level: 200
• potency: 300-400 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥95% spectrophotometric assay
• pH: 2.5 (25 °C, 10 g/L in H₂O)
• mp: 210 °C (decomposition)
• solubility: 100 g/L
• bulk density: 500‑600 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C27H33N2.H2O4S/c1-5-28(6-2)25-18-14-23(15-19-25)27(22-12-10-9-11-13-22)24-16-20-26(21-17-24)29(7-3)8-4;1-5(2,3)4/h9-21H,5-8H2,1-4H3;(H2,1,2,3,4)/q+1;/p-1
• InChI key: NNBFNNNWANBMTI-UHFFFAOYSA-M

Description

General description

Brilliant green (hydrogen sulfate) (C.I. 42040) for microscopy Certistain^®, is a dry staining dye that is used to prepare a staining solution makes target structures in bacteriological specimen materials evaluable for diagnostic purposes. In the area of bacteriology, among others the Gram staining method, the Ziehl-Neelsen staining method, and the spore-staining method according to Rakette are used to yield a differentiated image of pathogens. This can be done either by using eosin Y as a counterstain or by modification according to Wirtz using safranine O solution. By this method, spores are always stained green and the cell elements always red. As CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain^®

Analysis Note

Dye content (spectrophotometrically): ≥ 95 %
Identity
- Hydrogen sulfate (as sulfur):
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 628 - 632 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2210 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302 - H315 - H317 - H318 - H410
• pcodes: P261 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable