1.05397

GranuCult^® plus Malt extract broth

for yeasts, for molds, suitable for microbiology

• Synonym(s): Yeasts and molds medium
• EC Index Number: 232-310-9

Properties

• biological source: plant
• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Keep tightly closed.)
• technique(s): microbiological culture: suitable
• color: yellow-brown
• pH: 4.8 (25 °C, 17 g/L in H₂O, after autoclaving)
• solubility: 17 g/L
• bulk density: 590 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: molds; yeasts

Description

General description

GranuCult^® plus Malt extract broth contains polysaccharides which are used as an energy source and make the medium acidic. Mycological peptone serves as a nitrogen source. Lactic acid, tartaric acid, or antibiotics suppress bacterial growth and make the medium more selective. The pH value of the culture medium should be adjusted to 3.5 to obtain fungal counts and inhibit the growth of the bacterial flora. The malt extract broth can be modified for the selective cultivation of Aspergillus flavus. Furthermore, adding certain indicator dyes to malt extract agar allows the differentiation of yeast and bacterial colonies.

Application

Malt extract broth is suitable for the detection, isolation, and enumeration of fungi, particularly yeasts and molds, in various materials and for the cultivation of test strains for microbiological vitamin assays.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish brown
pH-value (25 °C): 4.6 - 5.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good to very good
Growth (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): good to very good
Growth (Saccharomyces cerevisiae ATCC 9080): good to very good
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Rhodotorula mucilaginosa DSM 70403): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): good to very good
Growth (Trichophyton ajelloi ATCC 28454): good to very good
Incubation: 7 days at 26 ± 2 °C, aerobic

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable