MilliporeSigma
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1.05616

Supelco

HPTLC plates, Silica gel F₂₅₄

pkg of 25 plates, plate L × W 5 cm × 10 cm, glass support

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material

glass support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 25 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

5 cm × 10 cm

particle size

5-6 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

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This Item
1.056291.056421.05628
feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

packaging

pkg of 25 plates

packaging

pkg of 100 plates

packaging

pkg of 50 plates

packaging

pkg of 25 plates

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

General description

25 Glass plates 5 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Linkage

Replaces: 105616-2; 105616

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steriod test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

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