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1.05642

Supelco

HPTLC plates, Silica gel 60 F₂₅₄

pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

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material

glass support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 50 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

20 cm × 10 cm

particle size

5-6 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

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This Item
1.056291.055561.05649
layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

pore size

-

packaging

pkg of 50 plates

packaging

pkg of 100 plates

packaging

pkg of 20 sheets

packaging

pkg of 25 plates

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

General description

50 Glass plates 20 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Application

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 20 x 10 cm, 50 sheets.

Linkage

Replaces: 5642-6; 5642

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

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Articles

Learn about our recently launched HPTLC calibration mix, developed in collaboration with CAMAG, that enables for an efficient and reliable universal system suitability testing (SST).

In this article we present several HPTLC applications and analytical standards for ginsenosides.

High-Performance Thin-Layer chromatography (HPTLC) quantification of methylglyoxal (MGO) in complex and matrix rich manuka honey offering quick sample preparation, high-matrix tolerance, and high-throughput.

Protocols

HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.

Powdered black pepper was chosen as example for dietary supplements, being a source for pierine. It is tested according to the current United States Pharmacopeia (USP) guidelines using HPTLC and HPLC.

In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.

Effective analysis of passion flower by HPTLC fingerprinting, in conjunction with a comprehensive range of related reference materials, enables fast and efficient identification of this plant material.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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