Documents

1.05642

Supelco

HPTLC Silica gel 60 F₂₅₄

pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

storage conditions

no restrictions.

material

glass support
silica gel 60 matrix

Quality Level

packaging

pkg of 50 plates

application(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

20 cm × 10 cm

storage temp.

2-30°C

General description

50 Glass plates 20 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Application

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 20 x 10 cm, 50 sheets.

Linkage

Replaces: 5642-6; 5642

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

Certificate of Analysis

Certificate of Origin

Articles
In this article we present several HPTLC applications and analytical standards for ginsenosides.
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HPTLC is an efficient method to determine Steviol Glycosides content in Stevia using our HPTLC plates and reference materials, including a new introduced Stevia extract reference material.
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Protocols
HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.
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Powdered black pepper was chosen as example for dietary supplements, being a source for pierine. It is tested according to the current United States Pharmacopeia (USP) guidelines using HPTLC and HPLC.
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In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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