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1.05719

Supelco

TLC plates, Silica gel 60 F₂₅₄

pkg of 200 plates, plate L × W 5 cm × 10 cm, glass support

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material

glass support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 200 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

250 μm

plate L × W

5 cm × 10 cm

particle size

10-12 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

200 Glass plates 5 x 10 cm
Our classical silica TLC plates are based on a combination of proven our silica gel 60 and the addition of a unique polymeric binder resulting in a very adherent and hard surface that will not crack or blister and even allow writing with a pensil on the surface without risk to damage the layer.

Application

Glass TLC plate, silica gel coated with flourescent indicator F254. The silica gel TLC plates come in a quantity of 200 size 5x10 cm for thin layer chromatography applications.

Linkage

Replaces: 5719-2; 5719

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.7 - 11.7 µm
Layer thickness: 210 - 270 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
11 - 25
- Ceres Black G, colour test, lipophile: 34 - 48
- ceres Violet brn, colour test, lipophile: 52 - 67
separation number (colourtest, lipophile): ≥ 9.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
25 - 37
- Reichstein S, steroid test: 37 - 49
- methyltestosterone, steroid test: 42 - 54
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

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