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1.05878

Millipore

GranuCult® MULLER-KAUFFMANN Tetrathionate Novobiocin broth (base)

according to ISO 6579, selective for Salmonella spp.

Synonym(s):
MKTTn broth, MKTT Novobiocin Supplement, Novobiocin Selective Supplement

Quality Level

Agency/Method

ISO 11133
according to ISO 6579

form

medium granules (dehydrated (DCM))

application(s)

microbiological culture: suitable
microbiology: suitable

Featured Industry

Food and Beverages

storage temp.

15-25°C

suitability

selective for Salmonella spp.

Related Categories

General description

MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth is a selective medium suitable for the enrichment and isolation of Salmonella. Tetrathionate in the broth suppresses the growth of coliform and other enteric bacteria. It is produced from thiosulfate by adding iodine to the culture medium. Salmonella, Proteus, and some other species of bacteria can reduce tetrathionate. Calcium carbonate buffers the sulphuric acid, which is generated when tetrathionate is reduced. Bile promotes the growth of Salmonella, but largely inhibits the accompanying bacteria. Brilliant green and novobiocin suppress the growth of primarily Gram-positive bacteria.

Application

MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth (base) is used for the selective enrichment of Salmonella from food, animal feed, and other materials.

Analysis Note

Appearance (clearness): turbid with sediment
Appearance (colour): green
pH-value (25 °C): 7.8 - 8.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
MKTTn broth: 24 ± 3 hours at 37 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Articles

Salmonella Contamination and Testing

Salmonella pass through the entire food chain from animal feed, primary production, and into households or food-service establishments.

Protocols

ISO 6579-1 for Salmonella Detection in the Food Chain

Specifies a horizontal method for the detection of Salmonella spp. in the food production chain.

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