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GranuCult® RAPPAPORT-VASSILIADIS-Soya broth (base)

ISO 6579, for Salmonella spp.

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Synonym(s):
Salmonella Enrichment Broth acc. to Rappaport and Vassiliadis, RVS broth (base), Rappaport Vassiliadis Salmonella Enrichment Broth

Agency

ISO 6579

Quality Level

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

5.0-5.4 (25 °C, 41.8 g/L in H2O, after autoclaving)

bulk density

700 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Salmonella spp.

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This Item
1.108241.461811.05394
form

medium granules (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

form

liquid (ready-to-use)

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

manufacturer/tradename

-

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

direct inoculation: suitable, sterile filtration: suitable

technique(s)

microbiological culture: suitable

pH

5.0-5.4 (25 °C, 41.8 g/L in H2O, after autoclaving)

pH

7.0-7.4 (25 °C, 54.4 g/L in H2O, after autoclaving)

pH

5.2

pH

7.3 (37 °C, 45 g/L in H2O, after autoclaving)

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology
pathogen testing

application(s)

microbiology
pharmaceutical

application(s)

food and beverages
microbiology

General description

RVS (RAPPAPORT-VASSILIADIS-Soya) broth is a selective medium for the enrichment and isolation of Salmonella. It is a modification of the original Rappaport medium, where soya peptone is used instead of tryptone (peptone from casein) to improve recovery rates of Salmonella. The efficiency of RVS broth for selection of Salmonella is based on (a) the ability of Salmonella sp. to multiply at relatively high osmotic pressures, at relatively low pH values, at a high temperature, and with modest nutritional requirements and (b) the suppression of the toxic effect of malachite green towards Salmonellae in the presence of magnesium chloride.

Application

RVS (RAPPAPORT-VASSILIADIS-Soya) broth (base) is used for the selective enrichment of Salmonella from food and animal feed, water, and other materials.

Analysis Note

Appearance (clearness): clear
Appearance (colour): darkblue
pH-value (25 °C): 5.0 - 5.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): colourless with black centre
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
RVS broth: 24 ± 3 hours at 41.5 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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