1.07994

Bacillus acidoterrestris agar

GranuCult^® prime, according to IFU 12, APHA, granular, for Alicyclobacillus spp., pkg of 500 g

• Synonym(s): Alicyclobacillus selective agar, GranuCult^® BAT agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• agency: APHA; according to IFU 12
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• storage condition: protect from light (both UV and Sun light)
• technique(s): microbiological culture: suitable
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Alicyclobacillus spp. (spp.)

Description

General description

GranuCult^® BAT agar supports the growth of Alicyclobacillus spp. These are aerobic, Gram-positive spore-forming bacteria, with optimum growth at low pH and increased temperatures. Alicyclobacilli are spoilage organisms, especially affecting the quality of fruit juices.
GranuCult^® BAT agar contains glucose as a carbon and energy source. Yeast extract is the source of vitamins, particularly the B-group. Potassium dihydrogen phosphate acts as a buffering system. The low pH and the high incubation temperature inhibit the contaminating flora. This medium contains many trace elements, which supply the specific requirements of the Alicyclobacillus spp. agar is the solidifying agent however, the gel strength is weak due to low pH.

Application

GranuCult^® BAT agar is suitable for the enumeration, detection, and identification of Alicyclobacillus spp. from fruit juices, other beverages, and their ingredients, environmental samples, including process water and other materials in the area of food production and food handling.

Analysis Note

Appearance (clearness): slightly turbid to turbid
Appearance (color): yellowish
pH-value (25 °C): 3.9 - 4.1
Growth (Alicyclobacillus acidocaldarius DSMZ 446): +
Growth (Alicyclobacillus acidoterrestris DSMZ 2498): +
Growth (Alicyclobacillus cycloheptanicus DSMZ 4006): +
Growth (Alicyclobacillus hesperidium DSMZ 12766): +
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): -
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): -
Incubation: A.acidocaldarius 72 hrs.; 60 °C aerobic; other strains 72 hrs.; 45 °C.
Reference for Staph.aureus und E.coli blood agar (Incubation: 24 hrs.; 35 °C).

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3