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1.08380

2,3,5-Triphenyltetrazolium chloride

Color indicator. Supplement in culture media for the microbiological analysis of food and water samples

Synonym(s):

2,3,5-Triphenyltetrazolium chloride, TTC, TT, TPTZ, TTZ, Tetrazolium Red

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$72.30

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About This Item

Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
298-96-4
Molecular Weight:
334.80
NACRES:
NA.74
UNSPSC Code:
41106212
Beilstein/REAXYS Number:
3923356
MDL number:
MFCD00011963

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SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI key

PKDBCJSWQUOKDO-UHFFFAOYSA-M

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

assay

≥98% (calculated on dried substance, perchloric acid titration)

form

solid

pH

3.7 (20 °C, 10 g/L in H2O)

mp

243 °C (decomposition)

solubility

150 g/L

bulk density

230 kg/m3

application(s)

microbiology

storage temp.

2-30°C

Quality Level

200

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This Item
T88771777930-4890
2,3,5-Triphenyltetrazolium chloride Color indicator. Supplement in culture media for the microbiological analysis of food and water samples

Millipore

1.08380

2,3,5-Triphenyltetrazolium chloride

2,3,5-Triphenyltetrazolium chloride ≥98.0% (HPLC)

Sigma-Aldrich

T8877

2,3,5-Triphenyltetrazolium chloride

2,3,5-Triphenyl-tetrazolium chloride solution suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

Millipore

17779

2,3,5-Triphenyl-tetrazolium chloride solution

2,3,5-Triphenyltetrazolium chloride JIS special grade, 98.0-102.0%

Sigma-Aldrich

30-4890

2,3,5-Triphenyltetrazolium chloride

assay

≥98% (calculated on dried substance, perchloric acid titration)

assay

≥98.0% (HPLC)

assay

-

assay

98.0-102.0%

form

solid

form

powder

form

liquid

form

solid

pH

3.7 (20 °C, 10 g/L in H2O)

pH

-

pH

-

pH

-

mp

243 °C (decomposition)

mp

-

mp

-

mp

-

solubility

150 g/L

solubility

H2O: 50 mg/mL

solubility

-

solubility

-

bulk density

230 kg/m3

bulk density

-

bulk density

-

bulk density

-

Application

2,3,5-triphenyltetrazolium chloride (TTC) is an indicator recommended for the microbiological analysis of food.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance): ≥ 98 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λmax. (water): 245 - 248 nm
Spec. Absorptivity A 1%/1cm (λmax; 0,01 g/l; water; calculated on dried substance): ≥ 770
TLC-Test: passes test
Loss on drying (70 °C; ≤ 1 hPa): ≤ 0.5 %
Suitability for testing the germinability of seeds: passes test
Suitability for microbiology: passes test

General description

2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. Live microorganisms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 3

Certificates of Analysis (COA)

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