1.08488

Urea

for biochemistry

• Synonym(s): Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• UNSPSC Code: 12352111
• NACRES: NA.21
• EC Index Number: 200-315-5
• MDL number: MFCD00008022
• Beilstein/REAXYS Number: 635724

Properties

• Product Name: Urea, for biochemistry
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• vapor pressure: <0.1 hPa ( 20 °C)
• form: solid
• potency: 8471 mg/kg LD₅₀, oral (Rat); 8200 mg/kg LD₅₀, skin (Rat)
• technique(s): titration: suitable
• pH: 9 (20 °C, 100 g/L in H₂O)
• mp: 132-135 °C (lit.)
• solubility: 1000 g/L
• density: 1.335 g/mL at 25 °C (lit.)
• bulk density: 720‑760 kg/m³
• storage temp.: 10-30°C
• Quality Level: 100

Description

Analysis Note

Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance: Colorless crystals or white crystalline powder.
Appearance of solution (100 g/l, water): Clear and colorless.
Melting point (DSC (differential scanning calorimetry)): 132 - 135 °C
UV-Absorption (260 nm; 8 mol/l; 1 cm; water): ≤ 0.1
UV-Absorption (280 nm; 8 mol/l; 1 cm; water): ≤ 0.1
Cyanide (CN): ≤ 0.000001 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Fe (Iron): ≤ 0.00002 %
NH₄ (Ammonium): ≤ 0.00001 %
Biuret: ≤ 0.05 %
Sulfated ash (800 °C): ≤ 0.01 %

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable