All Photos(1)

108975

Sigma-Aldrich

Leupeptin, Hemisulfate, Microbial - CAS 103476-89-7 - Calbiochem

Leupeptin hemisulfate, CAS 103476-89-7, is a reversible inhibitor of trypsin-like proteases and cysteine proteases

Synonym(s):
Ac-LLR-CHO, ½H₂SO₄, Leupeptin, Hemisulfate, Microbial - CAS 103476-89-7 - Calbiochem
Empirical Formula (Hill Notation):
C20H38N6O4 · 0.5H2SO4
CAS Number:
Molecular Weight:
475.59
MDL number:

Quality Level

assay

≥90% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

color

white

solubility

water: 50 mg/mL

shipped in

ambient

storage temp.

−20°C

InChI

1S/C20H38N6O4/c1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22/h11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23)/p+1/t15-,16-,17-/m0/s1

InChI key

GDBQQVLCIARPGH-ULQDDVLXSA-O

General description

A reversible inhibitor of trypsin-like proteases and cysteine proteases. Also known to inhibit activation-induced programmed cell death and to restore defective immune responses of HIV+ donors. Also available as a 50 mM solution in H2O (Cat. No. 509281).
A reversible inhibitor of trypsin-like proteases and cysteine proteases. Also known to inhibit activation-induced programmed cell death and to restore defective immune response in HIV+ donors. Often used for the inhibition of endoproteinase Lys-C, kallikrein, papain, plasmin, thrombin, cathepsin B, and trypsin. Leupeptin does not inhibit chymotrypsin, elastase, renin, or pepsin. The recommended working range is 10-100 µM for most applications.

Packaging

50 mg in Glass bottle
100 mg in Plastic ampoule
5, 10, 25 mg in Plastic ampoule

Biochem/physiol Actions

Product does not compete with ATP.
Primary Target
trypsin- and cysteine-like proteases
Reversible: yes
Cell permeable: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Leu-Leu-arginal, hemisulfate

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Aqueous stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C.

Other Notes

Montenez, J.P., et al. 1994. Toxicol. Lett. 73, 201.
Sarin, A., et al. 1994. J. Immunol.153, 862.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service