Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

1.09203

Sigma-Aldrich

Giemsa′s azur-eosin-methylene blue

for microscopy

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41116130
NACRES:
NA.21

Quality Level

form

solid

IVD

for in vitro diagnostic use

bulk density

520 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

General description

Giemsa′s azur-eosin-methylene blue - for microscopy dye, is a dry dye that is used for the preparation of a staining solution. Giemsa′s staining is one of the standard procedures in histology, hematology, cytology and bacteriology. When used in hematological applications, Giemsa′s stain is frequently used in combination with other dye solutions, e. g. with May-Grünwald′s solution for Pappenheim (MGG) overview staining. The stain demonstrates various cells with their morphological features better than e.g. with H&E stain in histology. This staining solution generally stains the nuclei red, based on the molecular interaction between the Eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For microscopy. For the staining of blood and bone-marrow smears, paraffin sections and clinical-cytological specimens

Analysis Note

Identity (UV/VIS-Spectrum)passes testLoss on drying (105 °C)≤ 10 %Suitability for microscopy (Blood smear)passes testNucleivioletLymphocytes (Plasma)blueMonocytes (Plasma)grey bluebasophilic granulocytes (Granules)dark violeteosinophilic granulocytes (Granules)reddish to red-brownNeutrophilic granulocytes (Granules)red-violetThrombocytesvioletErythrocytesreddish-brownishBlood parasites (Nuclei)lightred

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service