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1.09217

Sigma-Aldrich

Gram′s safranine solution

for the Gram staining method

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Quality Level

form

liquid

IVD

for in vitro diagnostic use

transition temp

flash point 49 °C

density

0.98 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

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1 of 4

This Item
1.102181.0056777730
vibrant-m

1.09217

Gram′s safranine solution

vibrant-m

1.10218

Gram′s decolorizing solution

vibrant-m

1.00567

Lugol′s solution

vibrant-m

77730

Gram Staining Kit

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

-

density

0.98 g/cm3 at 20 °C

density

0.79 g/cm3 at 20 °C

density

1.02 g/cm3 at 20 °C

density

-

form

liquid

form

liquid

form

liquid

form

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

General description

This ready-to-use Gram′s safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution and appear pink to red.
A bottle with 500 ml Gram′s safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

120.2 °F

flash_point_c

49 °C


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