1.09261

Sigma-Aldrich

Lugol′s solution (diluted iodine-potassium iodide solution)

for the Gram staining method

Synonym(s):
Iodine-potassium iodide solution

storage conditions

Store at +15°C to +25°C.

Quality Level

form

liquid

IVD

for in vitro diagnostic use

application(s)

hematology: suitable
histology: suitable

pH

3.5 (20 °C in H2O)

bp

100 °C/1013 hPa

density

1.01 g/cm3 at 20 °C

Featured Industry

Clinical Testing
Diagnostic Assay Manufacturing

storage temp.

15-25°C

General description

In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution - an aniline dye - in the first step. After the treatment with Gram′s iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution and appear orange. A 500 mL PE-bottle of Gram′s Lugol′s solution is sufficient for staining up to 250 slides. Lugol’s solution is also a part of the Gram-color stain set. It is an IVD & CE product.

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