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1.09628

Millipore

GranuCult® Buffered Listeria Enrichment Broth (base)

pkg of 500 g, FDA (BAM), for Listeria spp.

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Synonym(s):
Listeria Enrichment Broth, Listeria Selective Broth

reg. compliance

FDA (BAM)

Quality Level

form

medium granules (dehydrated (DCM))

packaging

pkg of 500 g

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.1-7.5 (25 °C in H2O)

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Listeria spp. (spp.)

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This Item
1.105491.108241.10398
packaging

pkg of 500 g

packaging

-

packaging

-

packaging

pkg of 500 g

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

pH

7.1-7.5 (25 °C in H2O)

pH

7.1-7.5 (30 °C, 36.1 g/L in H2O, after autoclaving)

pH

7.0-7.4 (25 °C, 54.4 g/L in H2O, after autoclaving)

pH

7.0-7.4 (25 °C, 57.4 g/L in H2O)

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
microbiology
pathogen testing

General description

GranuCult® Buffered Listeria Enrichment Broth (base) is a modification of the formulation of Tryptic Soy Broth (CASO) with the addition of 6 g/L yeast extract and by increasing its buffering strength. Dextrose is the carbohydrate source. Sodium chloride maintains the osmotic balance of the medium. Phosphate acts as a buffer. Sodium pyruvate mediates the recovery of sub-lethally damaged Listeria spp. The addition of acriflavine, cycloheximide, and nalidixic acid inhibits the growth of the accompanying flora.

Application

GranuCult® Buffered Listeria Enrichment Broth (base) is suitable for the selective enrichment of Listeria spp.

Linkage

Replaces: 1.09628.5007

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.1 - 7.5
Inoculum on reference medium (Listeria monocytogenes ATCC 19114 (WDCM 00110)):
Inoculum on reference medium (Listeria monocytogenes ATCC 19111 (WDCM 00020)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)):
Colony count (Listeria monocytogenes ATCC 19114 (WDCM 00110)): ≥ 1E+08 CFU/ml
Colony count (Listeria monocytogenes ATCC 19111 (WDCM 00020)): ≥ 1E+08 CFU/ml
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 1E+08 CFU/ml
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): passes test
Incubation 48 h at 30 °C

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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