All Photos(1)

1.10218

Sigma-Aldrich

Gram′s decolorizing solution

for the Gram staining method

Quality Level

form

liquid

autoignition temp.

>425 °C

IVD

for in vitro diagnostic use

application(s)

hematology: suitable
histology: suitable

transition temp

flash point -10 °C

density

0.79 g/cm3 at 20 °C

Featured Industry

Clinical Testing
Diagnostic Assay Manufacturing

storage temp.

15-25°C

General description

Gram′s decolorizing solution - for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodine solution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution (Gram′s safranine solution (Product number 1.09217) and appear pink to red.
A bottle with 500 ml Gram′s decolorizing solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU).

Analysis Note

Suitability for microscopy: passes test
Staphylococcus aureus: dark blue-violet
E.coli: pink to red

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

14.0 °F

Flash Point(C)

-10 °C

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service