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MilliporeSigma

1.10275

Violet Red Bile Dextrose Agar

GranuCult® prime, according to ISO 21528, USP, EP, JP, APHA, granular, for enterobacteriaceae, pkg of 500 g

Synonym(s):

VRBG Agar. Violet Red Bile Glucose Agar. Crystal-violet neutral-red bile dextrose agar, VRBD-agar (Crystal-violet neutral-red bile dextrose agar) acc. to MOSSEL

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500 G

$205.00

$205.00


Estimated to ship onDecember 08, 2025

Consider alternative product 70189-500G

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

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agency

APHA
EP
JP
USP
according to ISO 21528

Quality Level

sterility

non-sterile

form

granular

packaging

pkg of 500 g

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

pH

7.3 (37 °C, 40 g/L in H2O, after autoclaving)

solubility

39.5 g/L

bulk density

720 kg/m3

application(s)

food and beverages
microbiology
pharmaceutical

storage temp.

15-25°C

suitability

enterobacteriaceae

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This Item
1.040301.460001.01406
suitability

enterobacteriaceae

suitability

Escherichia coli, coliforms

suitability

Escherichia coli, Salmonella spp., coliforms

suitability

coliforms

form

granular

form

medium granules (dehydrated (DCM))

form

plate (ready-to-use)

form

granular

agency

APHA, ISO 21528, USP, EP, JP

agency

FDA-BAM

agency

APHA, EP, ISO 21528, JP, USP

agency

APHA, FDA-BAM, ISO 4832

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

sterility

non-sterile

sterility

-

sterility

sterile

sterility

non-sterile

application(s)

food and beverages
microbiology
pharmaceutical

application(s)

food and beverages, microbiology

application(s)

microbiology
pharmaceutical
surface monitoring

application(s)

food and beverages
microbiology

General description

Violet Red Bile Dextrose Agar is a granulated selective medium recommended for detection and enumeration of Enterobacteriaceae, especially the bile tolerant gram-negative bacteria from non-sterile products and pharmaceutical preparations. The granules ensure rapid and uniform dissolution in water, prevent clumping of the medium, and inhalation of the airborne powder. Pancreatic digest of gelatin and yeast extract provide nitrogenous compounds and other nutrients essential for bacterial metabolism. This media is selective due to the presence of the inhibitors, bile salts, and crystal violet. Crystal violet inhibits gram-positive organisms. The neutral red indicator helps to detect dextrose fermentation. Dextrose fermenting strains produce red colonies with pink-red halos in the presence of neutral red. Sodium chloride maintains the osmotic equilibrium in the medium.

Application

Violet Red Bile Dextrose Agar is recommended for the detection and enumeration of Enterobacteriaceae from pharmaceutical products.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.

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