1.10338
Fractogel® EMD Chelate (M)
purified by immobilized metal affinity chromatography (IMAC)
Recommended Products
ligand
iminodiacetic acid
Quality Level
description
for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
sterility
sterile (Caustic Stable)
form
resin
purified by
immobilized metal affinity chromatography (IMAC)
manufacturer/tradename
Fractogel®
parameter
8 bar max. pressure
~240 cm/hr flow rate
matrix active group
methacrylate
wide
40-90 μm
capacity
~60 mg binding capacity( lysozyme/ml of resin)
transition temp
flash point 35 °C (calculated)
application(s)
recombinant protein
separation technique
affinity
storage temp.
2-30°C
Related Categories
General description
Features and Benefits
- Efficiently purify histidine-tagged recombinant proteins
- Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
- Ensure timely and successful startup and operation
- Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
- Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime
Packaging
- 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
- 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
- 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
- 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L
Analysis Note
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk_germany
WGK 1
flash_point_f
95.0 °F
flash_point_c
35 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service