1.10398

Millipore

GranuCult® FRASER broth (base)

According to ISO 11290 and APHA; For microbiology

Synonym(s):
Listeria Selective Enrichment Broth
Pricing and availability is not currently available.

storage conditions

Store at +15°C to +25°C.

Quality Level

Agency/Method

APHA
ISO 11290

form

medium granules (dehydrated (DCM))

mfr. no.

GRANUCULT®

application(s)

microbiological culture: suitable
microbiology: suitable
pathogen testing: suitable

pH

7.0-7.4 (25 °C, 57.4 g/L in H2O)

solubility

57.4 g/L

Featured Industry

Food and Beverages

suitability

Listeria spp.

General description

Granucult® FRASER broth (base) is suitable for the primary and secondary selective enrichment of Listeria species. The high nutrient content and the large buffer capacity creates optimum growth conditions for Listeria. Lithium chloride, nalidixic acid, and acriflavine hydrochloride in the medium largely inhibit the growth of accompanying bacteria. The detection of the β-D-glucosidase activity is possible by the addition of aesculin and ammonium iron (III) citrate forming a black complex of aesculin iron (III) ions. However, this reaction is not exclusive to Listeria sp. so that following EN ISO 11290-1, every primary and secondary enrichment in Fraser broth has to be sub-cultured on selective plating media. From Granucult® FRASER Broth (Base), the primary selective enrichment Half Fraser broth, as well as the secondary enrichment Fraser broth, can be prepared by the addition of Ammonium iron (III) citrate supplement (1.00092) and Selective Supplement (1.00093).

Application

GranuCult® FRASER broth (base) is suitable for the primary and secondary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Linkage

Replaces: 1.10398

Analysis Note

Appearance (clearness): clear to almost clear
Appearance (color): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Blacking (Listeria monocytogenes ATCC 13932 (WDCM 00021)): +
Blacking (Listeria monocytogenes ATCC 35152 (WDCM 00109)): +
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Escherichia coli ATCC 8739 (WDCM 00012)): -
Blacking (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Blacking (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Growth on ALOA medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies
Growth on ALOA medium(Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <100 colonien
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <100 colonies
Incubation:
Half-Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Fraser Broth: 48 ± 2 hours at 37 ± 1°C
Confirmation on ALOA medium: 44 ± 4 hours at 37 ±1°C
Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C

Legal Information

ALOA is a registered trademark of Biolife Italiana S.r.l.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Articles
Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS
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Protocols
In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.
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