1.10426
Chromocult® Coliform Agar
ISO 9308-1: 2014, For the simultaneous detection and enumeration of coliform bacteria and E. coli
Recommended Products
agency
AOAC® RI (approved as alternative method for processed foods)
EPA
ISO 9308-1: 2014
Quality Level
form
medium granules (dehydrated (DCM))
manufacturer/tradename
ChromoCult®
technique(s)
microbiological culture: suitable
pH
6.6-7.0 (25 °C, 26.5 g/L in H2O)
solubility
26.5 g/L
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
Escherichia coli
coliforms
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General description
Application
Analysis Note
Appearance (colour): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Enterobacter aerogenes ATCC 13048):
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Enterobacter aerogenes ATCC 13048):
Colony count (Citrobacter freundii ATCC 43864 (WDCM 00006)):
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Enterobacter aerogenes ATCC 13048): ≥ 70 %
Recovery on test medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≥ 70 %
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): partial inhibition
Growth (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): no limit
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): dark-blue to violet
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): dark-blue to violet
Colonies (Enterobacter aerogenes ATCC 13048 (WDCM 00175)): pink to red
Colonies (Citrobacter freundii ATCC 43864 (WDCM 00006)): pink to red
Colonies (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): colourless
Incubation: 21 ± 3 hours at 36 ± 2 °C
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar
Legal Information
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Warning
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Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
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WGK 2
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