1.10747

BPLS agar, modified

GranuCult^® prime, according to ISO 6579, APHA, granular, selective for Salmonella spp., pack of 500 g

• Synonym(s): Brilliant-green Phenol-red Lactose Sucrose Agar, modified
• NACRES: NA.74
• UNSPSC Code: 41106213
• Manufacturer/tradename: GranuCult^® prime
• Agency: APHA, according to ISO 6579
• Ph: 6.7-7.1 (37 °C, 52 g/L in H₂O, after autoclaving)
• Form: granular
• Suitability: selective for Salmonella spp. (spp.)
• Application(s): food and beverages; microbiology

Properties

• Quality Level: 200
• agency: APHA, according to ISO 6579
• sterility: non-sterile
• form: granular
• packaging: pack of 500 g
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• pH: 6.7-7.1 (37 °C, 52 g/L in H₂O, after autoclaving)
• solubility: 51.5 g/L
• bulk density: 650 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: selective for Salmonella spp. (spp.)

Description

General description

GranuCult^® BPLS agar (modified) contains Brilliant green that inhibits the growth of the accompanying Gram-positive microbial flora, Salmonella typhi, and Shigella. However, the lower concentration of brilliant green in the modified BPLS agar does not strongly inhibit other Salmonella spp. The medium contains both lactose and sucrose. The color change (red to yellow) in the pH indicator Phenol red indicates the degradation of lactose to acid. Salmonellae do not ferment either lactose or sucrose and appear as red colonies surrounded by a bright red zone. Furthermore, in contrast to BPL agar, the sucrose in the medium allows identification of accompanying, weakly lactose-positive or lactose-negative, but sucrose-positive microorganisms.

Application

GranuCult^® BPLS agar (modified) is a selective agar suitable for the isolation of salmonellae (except for S. typhi) from meat, meat products and other foodstuffs.

Analysis Note

Appearance (clearness): clear
Appearance (colour): red
pH-value (25 °C): 6.7 - 7.1
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 40 %
Recovery on test medium (Salmonella choleraesuis ATCC 13312): ≥ 40 %
Recovery on test medium (Salmonella enteritidis NCTC 5188 (WDCM 00119)): ≥ 40 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Recovery on test medium (Proteus hauseri ATCC 13315):
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 0.01 %
Recovery on test medium (Enterococcus faecalis ATCC 33186): ≤ 0.01 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≤ 0.01 %
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)): pink-red
Colony colour (Salmonella choleraesuis ATCC 13312): pink-red
Colony colour (Salmonella enteritidis NCTC 5188 (WDCM 00119)): pink-red
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Colony colour (Proteus hauseri ATCC 13315): yellow
Incubation: 24 hrs.; 35 °C; aerobic.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3