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GranuCult® prime Peptone salt solution (Maximum recovery diluent)

according to ISO 6887, according to ISO 8199, for heterotrophic bacteria

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Synonym(s):
MRD, Maximum recovery diluent, Peptone solution, Saline Peptone Solution, Saline Peptone Water, Sodium Chloride Peptone Solution, Sodium Chloride Peptone Water

Agency

according to ISO 6887
according to ISO 8199

Quality Level

form

medium granules (dehydrated (DCM))

packaging

pkg of 500 g

manufacturer/tradename

GranuCult® prime

storage condition

protect from light (Keep tightly closed and dry.)

technique(s)

microbiological culture: suitable

color

light brown

pH

6.8-7.2 (25 °C, 9.5 g/L in H2O, after autoclaving)

solubility

9.5 g/L

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

heterotrophic bacteria

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form

medium granules (dehydrated (DCM))

form

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form

-

form

-

packaging

pkg of 500 g

packaging

pkg of 5x960tips/cs

packaging

pkg of 5x1000tips/cs

packaging

pkg of 5x1000tips/cs

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

Axygen Inc T-200-C-L-R-S, Corning

manufacturer/tradename

Axygen Inc T-1000-C-L-R, Corning

manufacturer/tradename

Axygen Inc T-1000-C-L, Corning

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

-

technique(s)

-

pH

6.8-7.2 (25 °C, 9.5 g/L in H2O, after autoclaving)

pH

-

pH

-

pH

-

General description

GranuCult® prime Peptone salt solution, also known as Maximum Recovery diluent, is of isotonic strength to ensure the recovery of organisms from various sources. It combines the protective effect of peptone in the diluent with the osmotic support of physiological saline. Within 1-2 hours of dilution of the sample, there is no multiplication of organisms due to the low concentration of peptones. Peptone salt solution complies with the recommendations of ISO 6887 and the German 35 Lebensmittelgesetz (German food law). This diluent can be used as an alternative to RINGER solution for milk and liquid milk products, dried milk, cheese, butter, meat and meat products, ice cream, and chilled food based on milk.

Application

GranuCult® prime Peptone salt solution is suitable for the preparation of an isotonic diluent for maximum recovery of organisms, especially in milk and meat testing.

Features and Benefits

  • GranuCult® offers superior granulated culture media.
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions.
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (color): colourless to slightly colourless
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034)): 70 - 130 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): 70 - 130 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): 70 - 130 %
Incubation: 45 minutes up to 1 hour at 20 - 25 °C
Reference medium: Tryptic Soy Agar 18 -24 hours at 35 - 37 °C, aerobic

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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Protocols

Revised EN ISO 21528:2017 for Detection and Enumeration of Enterobacteriaceae in the Food Chain

The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.

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