MilliporeSigma

1.14894

Millipore

Fractogel® EMD SE Hicap (M)

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Synonym(s):
Fractogel® EMD SE Hicap (M)

ligand

(Sulfoethyl)

Quality Level

description

strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

sterility

sterile (Caustic Stable)

product line

Fractogel®

form

resin

specific analyte(s)

MAbs

parameter

220 cm/hr flow rate
8 bar max. pressure

matrix active group

methacrylate

mean particle size

40-90 μm

capacity

160 mg binding capacity (lysozyme/ml of resin)

transition temp

flash point 35 °C (calculated)

application(s)

recombinant protein
vaccine development

separation technique

cation exchange
strong cation exchange

storage temp.

2-30°C

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This Item
1.168821.168831.10338
Fractogel® EMD DEAE (M)

Millipore

1.16883

Fractogel® EMD DEAE (M)

parameter

220 cm/hr flow rate, 8 bar max. pressure

parameter

8 bar max. pressure

parameter

170 cm/hr flow rate, 8 bar max. pressure

parameter

8 bar max. pressure, ~240 cm/hr flow rate

matrix active group

methacrylate

matrix active group

methacrylate

matrix active group

methacrylate

matrix active group

methacrylate

capacity

160 mg binding capacity (lysozyme/ml of resin)

capacity

130 mg binding capacity (lysozyme/ml of resin)

capacity

100 mg binding capacity (BSA/mg of resin)

capacity

~60 mg binding capacity( lysozyme/ml of resin)

separation technique

cation exchange, strong cation exchange

separation technique

cation exchange, strong cation exchange

separation technique

weak anion exchange

separation technique

affinity

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

General description

Strong cation exchange Fractogel® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides

Features and Benefits

Fractogel® EMD SE Hicap (M) features include:

  • Tentacle technology provides high yield, selectivity, and focused elution
  • High loading capacity combined with high purity at lower elution volumes
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Packaging

  • 1.14894.0100: Fractogel® EMD SE Hicap (M) Resin 100ml
  • 1.14894.0010: Fractogel® EMD SE Hicap (M) Resin 10ml
  • 1.14894.0500: Fractogel® EMD SE Hicap (M) Resin 500ml
  • 1.14894.5000: Fractogel® EMD SE Hicap (M) Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.08 %
Cerium: ≤ 20 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 120 - 160 mg/ml
Functional test (b:a): ≤ 0.20
Functional test: Separation of chymotrypsinogen A and lysozyme

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

95.0 °F

Flash Point(C)

35 °C


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