Documents

1.15423

Supelco

TLC Silica gel 60 RP-18 F₂₅₄s

pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

storage conditions

no restrictions.

material

glass support
silica gel 60 matrix

Quality Level

packaging

pkg of 50 plates

application(s)

thin layer chromatography (TLC): suitable

layer thickness

250 μm

plate L × W

20 cm × 10 cm

storage temp.

2-30°C

General description

50 Glass plates 10 x 20 cm
RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Application

Glass TLC plates, RP-18 modified silica gel coated with flourescent indicator F254s. The RP-18 modified silica gel TLC plates come in a quantity of 50 size 10x20 cm for thin layer chromatography applications.

Linkage

Replaces: 15423-6; 15423

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
d 50 (laser diffraction, size distribution): 9.5 - 11.5 µm
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
Layer thickness: 200 - 270 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
30 - 38
B) Steroid test
- methyltestosterone, steroid test: 21 - 31
- Reichstein S, steroid test: 50 - 60
- hydrocortisone, steroid test: 70 - 80
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.