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Sigma-Aldrich

Coomassie Brilliant blue G 250 (C.I. 42655)

for electrophoresis

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Synonym(s):
Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain
Empirical Formula (Hill Notation):
C47H48N3NaO7S2
CAS Number:
Molecular Weight:
854.02
MDL number:
EC Index Number:
228-058-4

Quality Level

potency

>5000 mg/kg LD50, oral (Rat)

technique(s)

protein staining: suitable

pH

6.4 (20 °C, 10 g/L in H2O)

solubility

40 g/L

bulk density

520 kg/m3

storage temp.

no temp limit

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1

InChI key

CMGWKYXJIYYESN-UHFFFAOYSA-M

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This Item
B0770278151.12553
vibrant-m

B0770

Brilliant Blue G

vibrant-m

27815

Brilliant Blue G

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

no temp limit

storage temp.

room temp

storage temp.

-

storage temp.

2-30°C

solubility

40 g/L

solubility

H2O: 1 mg/mL

solubility

-

solubility

20 g/L

pH

6.4 (20 °C, 10 g/L in H2O)

pH

-

pH

-

pH

6.2 (25 °C, 10 g/L in H2O)

potency

>5000 mg/kg LD50, oral (Rat)

potency

-

potency

-

potency

>5000 mg/kg LD50, oral (Rat)

General description

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.

Application

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λmax. (buffer pH 7.0): 577 - 584 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for electrophoresis: passes test

Legal Information

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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