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Sigma-Aldrich

Phloxin B (C.I. 45410)

for microscopy Certistain®

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Synonym(s):
Phloxin B (C.I. 45410)
Empirical Formula (Hill Notation):
C20H2Br4Cl4Na2O5
CAS Number:
18472-87-2
Molecular Weight:
829.63
MDL number:
MFCD00070627
EC Index Number:
242-355-6

form

solid

potency

2870 mg/kg LD50, oral (Rat)

composition

Dye, ≥80% gravimetric

pH

9.7 (20 °C, 1 g/L in H2O)

solubility

100 g/L

bulk density

650 kg/m3

εmax

930-1400 at 546-550 nm in 50% ethanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2

InChI key

OOYIOIOOWUGAHD-UHFFFAOYSA-L

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General description

The dry dye Phloxin B (C.I. 45410) for microscopy Certistain®, is used for the preparation of a staining solution for cytoplasmic staining in histological sections of human origin. It is mainly used in Hemytoxylin and Eosin (H&E) overview staining that is routinely performed in histological laboratories. Eosin and Phloxine bind to the positively charged plasma proteins. The combination of both dyes results in a broader color range in the tissue. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (gravimetrically): ≥ 80 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 546 - 550 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; ethanol 50 %): 930 - 1400
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(C)

Not applicable

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