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Sigma-Aldrich

Crystal violet (C.I. 42555)

for microscopy Certistain®

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Synonym(s):
Crystal violet (C.I. 42555), Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
Molecular Weight:
407.98
MDL number:
EC Index Number:
208-953-6

Quality Level

form

solid

potency

420 mg/kg LD50, oral (Rat)

composition

Dye, ≥88% spectrophotometric assay

pH

2.5-3.5 (20 °C, 10 g/L in H2O)

mp

189-194 °C

solubility

10 g/L

density

1.19 g/cm3 at 20 °C

bulk density

220‑400 kg/m3

εmax

2050-2450 at 589-594 nm in water at 0.002 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

General description

Crystal violet (C.I. 42555) for microscopy Certistain®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The Certistain® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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