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1.16216

Sigma-Aldrich

Peroxidase

(from horse radish) 10000 U/vial for biochemistry EC 1.11.1.7

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Synonym(s):
Peroxidase
CAS Number:
EC Index Number:
232-668-6

biological source

horseradish

Quality Level

form

solid

pH

6.5-7.5 (20 °C, 10 g/L in H2O)

storage temp.

2-8°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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This Item
P8415P8375P6140
Sigma-Aldrich

1.16216

Peroxidase

Peroxidase from horseradish Type XII, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

P8415

Peroxidase from horseradish

Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

P8375

Peroxidase from horseradish

Sigma-Aldrich

P6140

Peroxidase from horseradish

form

solid

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

ammonium sulfate suspension

pH

6.5-7.5 (20 °C, 10 g/L in H2O)

pH

-

pH

-

pH

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

-

Quality Level

300

Quality Level

-

General description

Horseradish peroxidase (HRP) is a glycoprotein that belongs to the class of oxidoreductase of the peroxidases family. It is found in horseradish roots. HRP contains significant amounts of carbohydrates and photohemin IX as its prosthetic group.

Biochem/physiol Actions

Peroxidases catalyze the cleavage of peroxides by redox reactions. The active center includes heme, cysteine thiols, selenium, manganese, and other chemical moieties. Peroxidase isoenzymes are essential for oxidizing reactive oxygen species, innate immunity, hormone biosynthesis, and the pathogenesis of many diseases. They are generally used for sensors, drug delivery devices, environment protection, bioremediation, and energy production. Horseradish peroxidase (HRP) is the widely used oxidoreductase in colorimetric, fluorimetric, chemiluminescent, electrochemical, and immunoassays. Its concentration can be measured with O-phenylenediamine (OPD) and hydrogen peroxide. HRP serves as a tracer for immunocytochemistry and is widely used in antibody-enzyme conjugates.

Features and Benefits

  • Ideal for preparing small-sized antibody-enzyme conjugates
  • Enables better cellular penetration through low-molecular-weight conjugates
  • Small conjugate size ensures greater accessibility to antigenic structures within tissue sections
  • Stable even under the conditions employed for crosslinking

Analysis Note

Activity
- Hydrogen peroxide and guajacol; pH 7.0; 25 °C:
about 170 U/mg
: ≥ 10000 U/Glas
Catalase: ≤ 1 U/mg

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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