1.16883
Fractogel® EMD DEAE (M)
weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
Synonym(s):
Fractogel® EMD DEAE (M)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mL | Check Cart for Availability | $97.00 | |
| 100 mL | Check Cart for Availability | $730.00 | |
| 500 mL | Check Cart for Availability | $2,560.00 | |
| 5 L | Check Cart for Availability | Inquire |
About This Item
$97.00
Quality Level
ligand
diethylaminoethyl
product line
Fractogel®
form
resin
parameter
170 cm/hr flow rate, 8 bar max. pressure
matrix active group
methacrylate
mean particle size
40-90 μm
capacity
100 mg binding capacity (BSA/mg of resin)
transition temp
flash point 35 °C (calculated)
density
1.430 g/cm3
bulk density
1000 kg/m3
application(s)
gene therapy
mAb
vaccine development
separation technique
weak anion exchange
storage temp.
2-30°C
General description
Features and Benefits
- Excellent binding to large viruses and plasmid DNA
- Homogenous binding with high selectivity and purity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Packaging
- 1.16883.0100: Fractogel® EMD DEAE (M) Resin 100ml
- 1.16883.0010: Fractogel® EMD DEAE (M) Resin 10ml
- 1.16883.0500: Fractogel® EMD DEAE (M) Resin 500ml
- 1.16883.5000: Fractogel® EMD DEAE (M) Resin 5L
Analysis Note
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| separation technique weak anion exchange | separation technique weak anion exchange | separation technique cation exchange, strong cation exchange | separation technique cation exchange, weak cation exchange |
| ligand diethylaminoethyl | ligand diethylaminoethyl | ligand (Sulfoisobutyl) | ligand carboxymethyl |
| form resin | form resin | form resin | form resin |
| capacity 100 mg binding capacity (BSA/mg of resin) | capacity 100 mg binding capacity (BSA/mg of resin) | capacity 130 mg binding capacity (lysozyme/ml of resin) | capacity 100 mg binding capacity (lysozyme/ml of resin) |
| matrix active group methacrylate | matrix active group methacrylate | matrix active group methacrylate | matrix active group methacrylate |
| product line Fractogel® | product line Fractogel® | product line Fractogel® | product line Fractogel® |
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signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
95.0 °F
flash_point_c
35 °C
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1168835000 | 04022536168722 |
| 1168830100 | 04022536741949 |
| 1168830010 | 04022536168708 |
| 1168839999 | 04022536718118 |
| 1168830500 | 04022536168715 |