MilliporeSigma

1.16886

Millipore

Fractogel® EMD COO- (M)

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Synonym(s):
Fractogel® EMD COO⁻ (M)

ligand

carboxymethyl

Quality Level

description

weak cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

sterility

sterile (Caustic Stable)

product line

Fractogel®

form

resin

specific analyte(s)

MAbs

parameter

300 cm/hr flow rate
8 bar max. pressure

matrix active group

methacrylate

mean particle size

40-90 μm

capacity

100 mg binding capacity (lysozyme/ml of resin)

transition temp

flash point 35 °C (calculated)

density

1.43 g/cm3 at 20 °C

application(s)

recombinant protein
vaccine development

separation technique

cation exchange
weak cation exchange

storage temp.

2-30°C

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This Item
1.168821.168841.16883
Fractogel® EMD COO- (M)

Millipore

1.16886

Fractogel® EMD COO- (M)

Fractogel® EMD DMAE (M)

Millipore

1.16884

Fractogel® EMD DMAE (M)

Fractogel® EMD DEAE (M)

Millipore

1.16883

Fractogel® EMD DEAE (M)

parameter

300 cm/hr flow rate, 8 bar max. pressure

parameter

8 bar max. pressure

parameter

170 cm/hr flow rate, 8 bar max. pressure

parameter

170 cm/hr flow rate, 8 bar max. pressure

matrix active group

methacrylate

matrix active group

methacrylate

matrix active group

methacrylate

matrix active group

methacrylate

capacity

100 mg binding capacity (lysozyme/ml of resin)

capacity

130 mg binding capacity (lysozyme/ml of resin)

capacity

100 mg binding capacity (BSA/mg of resin)

capacity

100 mg binding capacity (BSA/mg of resin)

separation technique

cation exchange, weak cation exchange

separation technique

cation exchange, strong cation exchange

separation technique

weak anion exchange

separation technique

weak anion exchange

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

General description

Weak cation exchange Fractogel® resin featuring the tentacle technology, efficient for difficult aggregate removal

Features and Benefits

Fractogel® EMD COO- (M) enables:
  • Good resolution between monomers and aggregates
  • Efficient aggregate and HCP removal combined with high yield
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the degree of the ionization of the functional groups of the exchanger
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Packaging

  • 1.16886.0100: Fractogel® EMD COO- (M) Resin 100ml
  • 1.16886.0010: Fractogel® EMD COO- (M) Resin 10ml
  • 1.16886.0500: Fractogel® EMD COO- (M) Resin 500ml
  • 1.16886.5000: Fractogel® EMD COO- (M) Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.05 %
Cerium: ≤ 10 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.30
Functional test (c:d): ≤ 0.70
Functional test: Separation of chymotrypsinogen A, cytochrome C and lysozyme

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

95.0 °F

Flash Point(C)

35 °C


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