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1.25079

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Fractogel® EMD DEAE (M)

MiniChrom Fractogel® DEAE (M), volume 5 mL, prepacked column

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Synonym(s):
MiniChrom Column Fractogel® DEAE (M), 5 ml

ligand

diethyl-(2-hydroxypropyl)aminoethyl

Quality Level

material

polypropylene housing

product line

Fractogel®

form

prepacked column

parameter

≤8 bar pressure
~170 cm/hr max. flow rate

bed L

100 mm

bed volume

5 mL

column I.D.

8 mm

volume

5 mL

matrix active group

Methacrylate

mean particle size

40-90 μm

capacity

~100 mg, BSA binding capacity

application(s)

gene therapy
mAb
vaccine development

separation technique

anion exchange

storage temp.

15-25°C

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matrix active group

Methacrylate

matrix active group

Methacrylate

matrix active group

Polyvinyl Ether

matrix active group

Methacrylate

product line

Fractogel®

product line

Fractogel®

product line

Eshmuno®

product line

Fractogel®

material

polypropylene housing

material

polypropylene housing

material

polypropylene housing

material

polypropylene housing

ligand

diethyl-(2-hydroxypropyl)aminoethyl

ligand

(Trimethylammoniumethyl)

ligand

(Trimethylammoniumethyl)

ligand

diethyl-(2-hydroxypropyl)aminoethyl

form

prepacked column

form

prepacked column

form

prepacked column

form

prepacked column

General description

Weak cation exchange Fractogel® EMD DEAE (M) resin in a convenient pre-packed 5 ml column

Features and Benefits

MiniChrom Column Fractogel® DEAE (M), 5 ml enables:
  • Excellent binding to large viruses and plasmid DNA
  • Homogenous binding with high selectivity and purity
  • Lower elution volumes for the highest purity levels
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the degree of the ionization of the functional groups of the exchanger
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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