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1.25184

Millipore

Fractogel® EMD SO3⁻ (S) Resin

RoboColumn Fractogel® SO3 (S), volume 0.6 mL, prepacked column

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Synonym(s):
RoboCol Fractogel® SO3 (S), 0.6ml

ligand

(Sulfoisobutyl)

Quality Level

material

polypropylene housing

product line

Fractogel®

form

prepacked column

parameter

≤8 bar pressure
~80 cm/hr max. flow rate

column I.D. × L

5 mm × 30 mm

volume

0.6 mL

matrix active group

Methacrylate

mean particle size

20-40 μm

capacity

~150 mL, resin binding capacity

application(s)

recombinant protein
vaccine development

storage temp.

no temp limit

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This Item
1.251551.251381.25137
Fractogel® EMD TMAE (S) RoboColumn Fractogel® TMAE (S), volume 0.6 mL, prepacked column

Millipore

1.25138

Fractogel® EMD TMAE (S)

product line

Fractogel®

product line

Fractogel®

product line

Fractogel®

product line

Fractogel®

matrix active group

Methacrylate

matrix active group

Methacrylate

matrix active group

Methacrylate

matrix active group

Methacrylate

ligand

(Sulfoisobutyl)

ligand

iminodiacetic acid

ligand

(Trimethylammoniumethyl)

ligand

(Trimethylammoniumethyl)

form

prepacked column

form

prepacked column

form

prepacked column

form

prepacked column

parameter

≤8 bar pressure

parameter

≤8 bar pressure, ~240 cm/hr max. flow rate

parameter

≤8 bar pressure, ~80 cm/hr max. flow rate

parameter

≤8 bar pressure, ~180 cm/hr max. flow rate

General description

5 x 30 mm

Features and Benefits

RoboColumn Fractogel® SO3 (S), 0.6ml enables:
  • Efficient separation between monomer, dimer, and high molecular weight aggregates
  • High binding capacity
  • High resolution
  • Efficient polishing step
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (S) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Packaging

Plastic bag(s)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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