126621

Bovine Serum Albumin

Cohn Fraction, 30% Aqueous Solution

• Synonym(s): Albumin, Bovine Serum, Cohn Fraction, 30% Aqueous Solution
• CAS Number: 9048-46-8
• MDL number: MFCD00011078
• UNSPSC Code: 12352202
• NACRES: NA.25
• eCl@ss: 32160409
• Assay: ≥95% (agarose gel electrophoresis)
• Form: liquid
• Technique(s): MALDI-TOF: suitable

Properties

• Product Name: Albumin, Bovine Serum, Cohn Fraction, 30% Aqueous Solution,
• description: Merck USA index - 14, 8468
• Quality Segment: 100
• assay: ≥95% (agarose gel electrophoresis)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): MALDI-TOF: suitable
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Albumin, Bovine Serum, (BSA) Cohn Fraction is purified by cold ethanol precipitation (Cohn extraction) and by heat treatment to remove lipids and fatty acids. It is a single carbohydrate-free polypeptide and the most abundant (comprising ~60%) of the plasma proteins. BSA regulates the maintenance of osmotic pressure.

BSA belongs to the serum albumin family,[1] comprising three domains with two sub-domains under each. BSA is an α-helical, globular, and non-glycosylated protein with 17-disulfide bonds.[2][1]

Biochem/physiol Actions

Bovine serum albumin (BSA) transports sparingly soluble physiological substances, especially long-chain fatty acids, bilirubin, drugs, hormones, and fatty acids.[2] It acts as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[3] BSA is a vital component of the cell culture media and boosts embryonic stem cells (hESC) differentiation.[4]

Physical form

30% solution in 0.8% NaCl, 0.1% NaN₃, pH 7.2, sterile-filtered.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3