Product density and activity are reported in the batch-specific Certificate of Analysis (CofA). The specifications are as follows: density ≥ 1.000 g/ml and activity ≥ 0.750 U/ml.
126741
Alcalase® Enzyme, Bacillus licheniformis
Synonym(s):
Protease from Bacillus licheniformis, subtilisin A
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500 ML
$113.00
About This Item
Recommended Products
biological source
Bacillus licheniformis
Quality Level
form
liquid
specific activity
≥0.75 Anson units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
activity assay: suitable
color
dark brown
application(s)
life science and biopharma
shipped in
ambient
storage temp.
2-8°C
1 of 4
This Item | P4860 | P5380 | A3403 |
|---|---|---|---|
| biological source Bacillus licheniformis | biological source - | biological source Bacillus licheniformis | biological source - |
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| specific activity ≥0.75 Anson units/mL | specific activity ≥2.4 U/g | specific activity 7-15 units/mg solid | specific activity ≥500 units/mg protein (biuret) |
| application(s) life science and biopharma | application(s) - | application(s) - | application(s) - |
| form liquid | form aqueous solution | form lyophilized powder | form saline solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
General description
Alcalase® is a serine endopeptidase that consists primarily of subtilisin A.[1]
Application
Biochem/physiol Actions
Alcalase® is suitable for the hydrolysis of proteins with an optimal pH of 7.0-8.5. It is used as an additive in detergent, dehairing, and bating leather. Alcalase is also used in meat tenderizing, cheese flavor development, baked manufacture, and improving the digestibility of animal feeds. Commercial Alcalase® comprises a liquid enzymatic preparation containing 50% glycerol, 41% water, and 9% protease extract from Bacillus licheniformis.[1]
Warning
Toxicity: Harmful (C)
Unit Definition
One unit is defined as the amount of enzyme that will release 1.0 µmol L-tyrosine from hemoglobin per min at 25°C, pH 7.5.
Reconstitution
Please refer to the vial label for lot-specific density.
Legal Information
Alcalase is a registered trademark of Novozymes Corp.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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What is the density or the concentration of the enzyme because I can't find it in the FDS ?
1 answer-
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Can I dilute the enzyme in distilled/deionized water or should I use any specific buffer for dilution?
1 answer-
Double distilled water can be used to further dilute product 126741.
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