14-392

Green Fluorescent Protein

• Synonym(s): GFP
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• mol wt: M_w ~28 kDa
• Quality Level: 100
• packaging: pkg of 300 μg
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• GenBank® accession no.: AAA27722
• UniProt accession no.: P42212
• shipped in: dry ice

Description

General description

N-terminal HIS-tagged fusion protein corresponding to full-length Green Fluorescent Protein, expressed in E. coli. Green fluorescent protein (GFP) is a cytoplasmic protein found in jellyfish Aequorea Victoria.

Biochem/physiol Actions

Green fluorescent protein (GFP) is genetically fused to many proteins in various species to generate non-reactive chimeras which are known to preserve their original biological activity as well as the fluorescent properties of native GFP. GFP acts as a reporter for gene expression and as a fusion tag for protein localization studies in live cells. It serves as a spectroscopic and microscopic probe to understand the biological functions of various systems. GFP is highly stable and can serve as a tool for in vitro studies.

Quality

Routinely evaluated as a standard on protein gels and western immunoblots, for calibration of fluorometers, and microinjection into cells and tissues.

Physical form

PBS containing 20% glycerol.

Storage and Stability

1 year at -20°C

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable