14-475-M

Cdk2/Cyclin E Protein, active, 10 µg

Active complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• shelf life: 1 yr
• mol wt: M_w 34 kDa (cdk2); M_w 74 kDa (cyclin)
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• solubility: water: soluble
• NCBI accession no.: NM_001798.2; NM_052827.1
• UniProt accession no.: P24941
• Gene Information: human ... CCNE1(898), CDK2(898)

Description

General description

Research area: APOPTOSIS

Complex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag. Product Source: Both are expressed by baculovirus in Sf21 insect cells The Cyclin E genes (CCNE1) gene located at 19q12 region in humans is responsible for encoding full-length protein of 410 amino acids.[1]

Biochem/physiol Actions

Protein Target: CDK2/cyclinE

Target Sub-Family: CMGC

The Cyclin E1 protein binds to and activates Cyclin-dependent kinase 2 (CDK2), forming an active complex crucial for the initiation and progression of the S phase. Cyclin E/CDK2 complex primarily regulates cell cycle advancement and DNA replication by phosphorylating specific substrates. Elevated Cyclin E protein levels and heightened CDK2 kinase activity are both individually linked to unfavorable prognosis, decreased survival, and resistance to therapy in cancer patients. Overactivation of the Cyclin E/CDK2 complex directly disrupts DNA replication through multiple mechanisms, resulting in DNA double strand breaks (DSBs) and genomic instability. Targeting the oncogenic Cyclin E/CDK2 complex has emerged as a potential therapeutic approach against cancer.[1]

Packaging

Also available in 250μg size as catalog # 14-475M - Call for pricing and availability.

Quality

routinely evaluated by phosphorylation of histone H1 by cdk2/cyclin E

Physical form

Ni-NTA agarose

Storage and Stability

1year at -70C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.