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14-484-M

Sigma-Aldrich

PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

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Synonym(s):
PRKCA, Protein kinase C alpha type
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

shelf life

1 yr

mol wt

Mw 78 kDa

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

accession no.

NM_002737.2

NCBI accession no.

UniProt accession no.

storage temp.

−70°C

Gene Information

human ... PRKCA(5578)

General description

Research area: Apoptosis

Protein Target: PKCα

Target Sub-Family: AGC
Recombinant, full-length human PKCα, containing N-terminal His6 tag

Application

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

Biochem/physiol Actions

Protein kinase C-α (PKCα), an isoform of protein kinase C, exhibits an anti-apoptotic and pro-proliferative function. It is commonly upregulated in tumor cells, and its depletion has been associated with apoptosis induction. The anti-apoptotic effects of PKCα are mediated through mechanisms such as phosphorylation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and activation of the pro-survival protein kinase AKT. The inhibition of PKCα leads to the activation of a PKCδ-dependent apoptotic pathway in salivary epithelial cells. PKCα regulates cell cycle progression in glioma cells by increasing the expression of p21 cyclin-dependent kinase inhibitor 1 (p21 (Waf1/Cip1)), a protein involved in cell cycle regulation.

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of Histone H1.

Target description

PKCα

Physical form

Ni2+/NTA-agarose

Storage and Stability

1 year at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_c

Not applicable


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