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HEIMPLATE Mannitol Salt Agar

90 mm settle plate with 30 ml filling volume for isolation and presumptive identification of Staphylococcus aureus in non-sterile pharmaceuticals - Long incubation

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Synonym(s):
MSA, Chapman Medium

agency

EP
JP
USP

Quality Level

sterility

sterile

form

plate (ready-to-use)

feature

ready-to-use

packaging

pkg of (20 or 120 plates per box)

technique(s)

microbiological culture: suitable

pH

7.4 ( in H2O)

application(s)

microbiology
pharmaceutical

storage temp.

15-25°C

suitability

Staphylococcus spp.

Related Categories

General description

Mannitol Salt Agar is a modified version of the selective agar proposed by Chapman (1945) for the isolation and presumptive identification of Staphylococcus aureus. The medium supports the growth of Staphylococcus aureus, whereas its high salt content (7.5%) inhibits the growth of many other microorganisms. In contrast to most other Staphylococci, coagulase-positive S. aureus can build acids from mannitol. Therefore, they build up yellow colonies with yellow zones. Beef extract, a pancreatic digest of casein, and animal tissue in the media provide carbon, nitrogen compounds, long-chain amino acids, vitamins, trace nutrients and other essential growth factors. Phenol red indicator aids in the detection of acid produced by fermentation of mannitol.

Application

The Mannitol Salt Agar media is recommended for the isolation and presumptive identification of Staphylococcus aureus in non-sterile pharmaceuticals and foods. However, the coagulase test will need to confirm the findings.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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