1.46597

PPLO Broth

bottle capacity 1000 mL, bottle filling volume 500 mL, closure type, Blue screw cap with 3 septa, pack of 6 bottles, selective for mycoplasma

• Synonym(s): Mycoplasma Broth, Pleuropneumonia-like organisms (PPLO) Broth
• UNSPSC Code: 12352201

Properties

• agency: EP 2.6.1; JP 4.06; USP 71; 71
• Quality Level: 300
• sterility: sterile; autoclaved
• form: liquid
• feature: closure type Blue screw cap with 3 septa; ready-to-use
• packaging: pack of 6 bottles
• technique(s): direct inoculation: suitable
• bottle capacity: 1000 mL
• bottle filling volume: 500 mL
• pH: 7.8± 0.2
• application(s): cosmetics; food and beverages; pharmaceutical; sterility testing
• storage temp.: 2-25°C
• suitability: selective for mycoplasma

Description

General description

PPLO Broth is a nutrient rich mycoplasma detection media. Mycoplasmas are the smallest bacteria besides nanobacteria. Due to the lack of cell wall structures they pass filters with pore sizes of 0.2 μm and show a great sensitivity towards osmotic changes within their habitats. They grow aerobically or under facultative anaerobic conditions.
This medium contains beef heart infusion and peptone to supply nutrients required for the growth of mycoplasmas. Sodium chloride maintains osmotic equilibrium. Yeast extract supplies a variety of B-complex vitamins and enhances growth. Normal horse serum provides a protein source. Penicillin and thallium acetate are added as inhibitors of bacteria. Yeasts and molds are inhibited by the amphotericin B contained in the medium. 

Application

PPLO broth is used to detect presence of mycoplasma in test samples through direct inoculation or sterile filtration methods.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable