MilliporeSigma
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14-811

Sigma-Aldrich

UbcH5c Conjugating Enzyme, 100 µg

The His6-tagged fusion protein of UbcH5c is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.

eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

assay

>95% (SDS-PAGE)

mol wt

Mw 17 kDa

manufacturer/tradename

Upstate®

UniProt accession no.

shipped in

dry ice

General description

Product Source: Recombinant human UbcH5c Conjugating Enzyme expressed in E. coli.
The His6-tagged fusion protein of UbcH5c is charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation. UbcH5c is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53, NF-κB, and c-Fos. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions.

Storage and Stability

Stable for 1 year at -80°C from date of shipment. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificate of Quality

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