14-813

Sigma-Aldrich

UbcH7 Conjugating Enzyme, 100 µg

UbcH7 is a class I enzyme which functions in the stress response & the control of transcription factors. The enzyme is ubiquitously expressed with high levels of expression seen in adult muscle.

eCl@ss:
32160405
NACRES:
NA.32

Quality Level

biological source

human

assay

>95% (SDS-PAGE)

mol wt

Mw 18 kDa

manufacturer/tradename

Upstate®

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... UBE2G2(7327)

General description

UbcH7 is a class I enzyme which functions in the stress response and the control of transcription factors. The enzyme is ubiquitously expressed with high levels of expression seen in adult muscle. It has been demonstrated to participate in the ubiquitinylation of p53, c-Fos and NF-κB. UbcH7 is one of two E2s (UbcH5 being the other) with which HECT domain proteins interact with UbcH7 being able to efficiently substitute for UbcH5 in E6-AP-dependent ubiquitinylation. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions.
Product Source: Recombinant human UbcH7 Conjugating Enzyme expressed in E. coli.

Physical form

Purified using Ni2+ chelation chromatography with Ni2+ in limiting concentration.

Storage and Stability

Stable for 1 year at -80°C from date of shipment. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
K K Chung et al.
Nature medicine, 7(10), 1144-1150 (2001-10-09)
Parkinson disease is a common neurodegenerative disorder characterized by the loss of dopaminergic neurons and the presence of intracytoplasmic-ubiquitinated inclusions (Lewy bodies). Mutations in alpha-synuclein (A53T, A30P) and parkin cause familial Parkinson disease. Both these proteins are found in Lewy...
U Nuber et al.
The Journal of biological chemistry, 271(5), 2795-2800 (1996-02-02)
E6-AP, a 100-kDa cellular protein, was originally identified through its interaction with the E6 protein of the oncogenic human papillomavirus types 16 and 18. The complex of E6-AP and E6 specifically interacts with p53 and mediates ubiquitination of p53 in...

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